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1.
A. Lapolla C. Gerhardinger L. Baldo D. Fedele A. Keane R. Seraglia S. Catinella P. Traldi 《生物化学与生物物理学报:疾病的分子基础》1993,1225(1):33-38
The number of glucose molecules condensed on glycated bovine serum albumin have been easily determined by means of matrix-assisted laser desorption/ionization mass spectrometry. Measurements were carried out on samples from incubation of the proteins with glucose at different concentrations (0.02 M, 0.2 M, 2 M and 5 M). A clear increase in molecular mass of BSA with respect to incubation time is detected. In contrast to what is observed with fluorescence, the plots of molecular mass increase vs. incubation time show tha occurrence of a steady state, corresponding to the complete saturation of all the protein sites against glucose. Comparison of fluorescence and molecular mass data reveals that some further reactions, different from condensation, must take place, which could be in principle either intramolecular or originated by reactivity of modified condensed gluocse moieties vs. free glucose. 相似文献
2.
E. S. Jensen 《Plant and Soil》1991,133(1):83-92
Simultaneous determination of 15N and total N using an automated nitrogen analyser interfaced to a continuous-flow isotope ratio mass spectrometer (ANA-MS method) was evaluated. The coefficient of variation (CV) of repeated analyses of homogeneous standards and samples at natural abundance was lower than 0.1%. The CV of repeated analyses of 15N-labelled plant material and soil samples varied between 0.3% and 1.1%. The reproductibility of repeated total N analyses using the automated method was comparable to results obtained with a semi-micro Kjeldahl procedure. However, the automated method gave results which were 3% to 5% higher than those obtained with the Kjeldahl procedure. Since only small samples can be analysed, careful sample homogenization and fine grinding are very important. Evaluation of a diffusion method for preparing nitrate and ammonium in solution for automated 15N analysis showed that the recovery of inorganic N in the NH3 trap was lower when the N was diffused from water than from 2 M KCl. The results also indicated that different proportions of the NO3
- and the NH4
+ in aqueous solution were recovered in the trap after combined diffusion. The method is most suited for diffusing either NO3
- or NH4
+ alone, but can be used for combined diffusion of the two ions. 相似文献
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E. Golomer P. Dupui H. Monod 《European journal of applied physiology and occupational physiology》1997,76(2):140-144
We investigated the effects of maturation on the dynamic body sways of healthy girls. Prepubertal and postpubertal girls
practising professional physical activities requiring a good ability to maintain equilibrium (acrobats and dancers) were asked
to stand on a free seesaw platform and the results compared to those for untrained age-matched girls. This platform (stabilometer)
allows self-induced body sways. Stabilograms were obtained by a double integration of the angular acceleration from the recordings
of the platform sways made with an accelerometer. Fast Fourier transform processing of stabilograms allowed spectral frequency
analysis. The total spectrum energy and the energies of three frequency bands (0–0.5 Hz, 0.5–2 Hz, 2–20 Hz) were determined.
ANOVA showed that, for all groups of different equilibrium activity and independent of visual input, prepubertal girls had
higher energy values than postpubertal girls in the 0- to 0.5-Hz band whereas the opposite was true for 0.5- to 2-Hz band.
Ballet dancers were more dependent than acrobats on visual inputs for the regulation of their postural control but were less
dependent than untrained girls at both ages. Maturation seemed to shift body sways towards higher frequencies and the utilization
of the cues of postural control was different according to the type of equilibrium activity practised by the subjects.
Accepted: 7 February 1997 相似文献
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Cation export from Alaskan arctic watersheds 总被引:1,自引:1,他引:0
Jeffrey C. Cornwell 《Hydrobiologia》1992,240(1-3):15-22
The concentrations and stream fluxes of Na, K, Ca and Mg were determined in 1978, 1980 and 1981 in the Toolik Lake watershed
and in 1978 in the upper Kuparuk River watershed. The annual export of K was strongly influenced by high K concentrations
at the initiation of spring melt. Potassium concentrations were positively correlated with concentrations of particulate and
dissolved organic carbon, particulate and dissolved phosphorus, and particulate, dissolved organic and ammonium nitrogen,
suggesting a common origin in the decomposition of tundra plants. Calcium and Mg stream concentrations were generally highest
in July and August when the depth of the soil active layer reached a maximum of ∼ 0.5 m. Precipitation-derived Na and K accounted
for up to half of the Na and K stream export while chemical weathering supplied > 85 % of exported Ca and Mg. Cation export
rates in these arctic foothill watersheds are among the lowest recorded. 相似文献
9.
《Molecular & cellular proteomics : MCP》2023,22(1):100476
Cancer-derived extracellular vesicles (EVs) promote tumorigenesis, premetastatic niche formation, and metastasis via their protein cargo. However, the proteins packaged by patient tumors into EVs cannot be determined in vivo because of the presence of EVs derived from other tissues. We therefore developed a cross-species proteomic method to quantify the human tumor-derived proteome of plasma EVs produced by patient-derived xenografts of four cancer types. Proteomic profiling revealed individualized packaging of novel protein cargo, and machine learning accurately classified the type of the underlying tumor. 相似文献
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